The Wzi outer membrane protein mediates assembly of a tight capsular polysaccharide layer on the Acinetobacter baumannii cell surface
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Description
Identification of novel therapeutic targets is required for developing alternate strategies to treat infections caused by the extensively drug-resistant bacterial pathogen, Acinetobacter baumannii. As capsular polysaccharide (CPS) is a prime virulence determinant required for evasion of host immune defenses, understanding the pathways for synthesis and assembly of this discrete cell-surface barrier is important. In this study, we assess cell-bound and cell-free CPS material from A. baumannii AB5075 wildtype and transposon library mutants and demonstrate that the Wzi outer membrane protein is required for the proper assembly of the CPS layer on the cell surface. Loss of Wzi resulted in an estimated 4.4-fold reduction in cell-associated CPS with a reciprocal increase in CPS material shed in the extracellular surrounds. Transmission electron microscopy revealed a disrupted CPS layer with sparse patches of CPS on the external face of the outer membrane when Wzi function was lost. However, this genotype did not have a significant effect on biofilm formation. Genetic analysis demonstrated that the wzi gene is ubiquitous in the species, though the nucleotide sequences were surprisingly diverse. Though divergence was not concomitant with variation at the CPS biosynthesis K locus, an association between wzi type and the first sugar of the CPS representing the base of the structure most likely to interact with Wzi was observed.
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ID Code: | 226709 | ||||||
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Item Type: | Contribution to Journal (Journal Article) | ||||||
Refereed: | Yes | ||||||
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Additional Information: | Funding Information: J.J.K. is supported by an Australian Research Council (ARC) DECRA fellowship (DE180101563) and S.H. by an Australian Government Research Training Program (RTP) Scholarship. This project was further supported by funding from the Advance QLD Women’s Research Assistance Program (WRAP067-2019RD1) and in part by National Health Medical Research Council Project Grant GNT1144046 to M.T. | ||||||
Measurements or Duration: | 12 pages | ||||||
DOI: | 10.1038/s41598-021-01206-5 | ||||||
ISSN: | 2045-2322 | ||||||
Pure ID: | 102082793 | ||||||
Divisions: | Current > Research Centres > Centre for Immunology and Infection Control Current > QUT Faculties and Divisions > Faculty of Health Current > Schools > School of Biomedical Sciences |
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Funding Information: | J.J.K. is supported by an Australian Research Council (ARC) DECRA fellowship (DE180101563) and S.H. by an Australian Government Research Training Program (RTP) Scholarship. This project was further supported by funding from the Advance QLD Women’s Research Assistance Program (WRAP067-2019RD1) and in part by National Health Medical Research Council Project Grant GNT1144046 to M.T. | ||||||
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Copyright Owner: | 2021 The Author(s) | ||||||
Copyright Statement: | This work is covered by copyright. Unless the document is being made available under a Creative Commons Licence, you must assume that re-use is limited to personal use and that permission from the copyright owner must be obtained for all other uses. If the document is available under a Creative Commons License (or other specified license) then refer to the Licence for details of permitted re-use. It is a condition of access that users recognise and abide by the legal requirements associated with these rights. If you believe that this work infringes copyright please provide details by email to qut.copyright@qut.edu.au | ||||||
Deposited On: | 01 Dec 2021 04:40 | ||||||
Last Modified: | 28 Mar 2024 20:28 |
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