Structure of the K87 capsular polysaccharide and KL87 gene cluster of Acinetobacter baumannii LUH5547 reveals a heptasaccharide repeating unit
|
Accepted Version
(PDF 1MB)
104905557. Available under License Creative Commons Attribution Non-commercial No Derivatives 4.0. |
Description
K87 capsular polysaccharide (CPS) was isolated from Acinetobacter baumannii isolate LUH5547 that carries the KL87 capsule biosynthesis gene cluster at the chromosomal K locus. Studies by sugar analysis, selective chemical cleavages, and 1D and 2D 1H and 13C NMR spectroscopy showed that the CPS has a branched heptasaccharide repeat (K unit) containing one residue each of D-glucose (D-Glсp), D-glucuronic acid (D-GlсpA), N-acetyl-D-glucosamine (D-GlсpNAc), 6-deoxy-L-talose (L-6dTalp), and three residues of L-rhamnose (L-Rhap). The following structure of the CPS was established:
→3)−α-L-Rhap−(1→2)-α-L-Rhap-(1→3)-α-L-6dTalp-(1→3)-β-D-GlcpNAc-(1→
2
↑
1
β-D-GlcpA-(4←1)-α-D-Glcp(2←1)-α-L-Rhap
The position of a minor O-acetyl group present in the CPS was not determined. Functions of enzymes coded by genes in the KL87 gene cluster were tentatively assigned and found to be consistent with the CPS structure.
Impact and interest:
Citation counts are sourced monthly from Scopus and Web of Science® citation databases.
These databases contain citations from different subsets of available publications and different time periods and thus the citation count from each is usually different. Some works are not in either database and no count is displayed. Scopus includes citations from articles published in 1996 onwards, and Web of Science® generally from 1980 onwards.
Citations counts from the Google Scholar™ indexing service can be viewed at the linked Google Scholar™ search.
Full-text downloads:
Full-text downloads displays the total number of times this work’s files (e.g., a PDF) have been downloaded from QUT ePrints as well as the number of downloads in the previous 365 days. The count includes downloads for all files if a work has more than one.
ID Code: | 227490 | ||
---|---|---|---|
Item Type: | Contribution to Journal (Journal Article) | ||
Refereed: | Yes | ||
ORCID iD: |
|
||
Additional Information: | Funding Information: This work was supported by the Russian Science Foundation (project No.19-14-00273) to Y.A.K., an Australian Research Council (ARC) DECRA Fellowship DE180101563 to J.J.K, and an NHMRC Investigator grant ( GNT1194178 ) to R.M.H. | ||
Measurements or Duration: | 8 pages | ||
Keywords: | 6-Deoxy-L-talose, Acinetobacter baumannii, Capsular polysaccharide, K locus, K87 | ||
DOI: | 10.1016/j.carres.2021.108439 | ||
ISSN: | 0008-6215 | ||
Pure ID: | 104905557 | ||
Divisions: | Current > Research Centres > Centre for Immunology and Infection Control Current > QUT Faculties and Divisions > Faculty of Health Current > Schools > School of Biomedical Sciences |
||
Funding Information: | This work was supported by the Russian Science Foundation (project No.19-14-00273) to Y.A.K., an Australian Research Council (ARC) DECRA Fellowship DE180101563 to J.J.K, and an NHMRC Investigator grant ( GNT1194178 ) to R.M.H. | ||
Funding: | |||
Copyright Owner: | 2021 Elsevier Ltd | ||
Copyright Statement: | This work is covered by copyright. Unless the document is being made available under a Creative Commons Licence, you must assume that re-use is limited to personal use and that permission from the copyright owner must be obtained for all other uses. If the document is available under a Creative Commons License (or other specified license) then refer to the Licence for details of permitted re-use. It is a condition of access that users recognise and abide by the legal requirements associated with these rights. If you believe that this work infringes copyright please provide details by email to qut.copyright@qut.edu.au | ||
Deposited On: | 20 Jan 2022 04:08 | ||
Last Modified: | 03 Jun 2024 07:11 |
Export: EndNote | Dublin Core | BibTeX
Repository Staff Only: item control page