The K46 and K5 capsular polysaccharides produced by Acinetobacter baumannii NIPH 329 and SDF have related structures and the side-chain non-ulosonic acids are 4-O-acetylated by phage-encoded O-acetyltransferases
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Description
Acinetobacter baumannii isolate NIPH 329 carries a novel capsular polysaccharide (CPS) gene cluster, designated KL46, that is closely related to the KL5 locus in A. baumannii isolate SDF but includes genes for synthesis of 5,7-diacetamido-3,5,7,9-tetradeoxy-l-glycero-l-manno-non-2-ulosonic (di-N-acetylpseudaminic) acid (Pse5Ac7Ac) instead of the corresponding D-glycero-D-galacto isomer (di-N-acetyllegionaminic acid) (Leg5Ac7Ac). In agreement with the genetic content of KL46, chemical studies of the K46 CPS produced by NIPH 329 revealed a branched tetrasaccharide repeat (K unit) with an overall structure the same as K5 from SDF but with â-Pse5Ac7Ac replacing α-Leg5Ac7Ac. As for K5, the K46 unit begins with d-GalpNAc and includes α-d-GlcpNAc-(1→3)-d-GalpNAc and α-d-Galp-(1→6)-d-GlcpNAc linkages, formed by Gtr14 and Gtr15 glycosyltransferases, respectively. The Gtr94K46 glycosyltransferase, which is related to Gtr13K5, links Pse5Ac7Ac to d-Galp in the growing K unit via a â-(2→6) linkage. Nearly identical Wzy enzymes connect the K46 and K5 units via a α-D-GalpNAc-(1→3)-α-D-Galp linkage to form closely related CPSs. Both Pse5Ac7Ac in K46 and Leg5Ac7Ac in K5 are acetylated at O4 but no acetyltransferase gene is present in KL46 or KL5. Related acetyltransferases were found encoded in the NIPH 329 and SDF genomes, but not in other strains carrying an unacetylated Pse or Leg derivative in the CPS. The genes encoding the acetyltransferases were in different putative phage genomes. However, related acetyltransferases were rare among the >3000 publically available genome sequences.
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| ID Code: | 227503 | ||
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| Item Type: | Contribution to Journal (Journal Article) | ||
| Refereed: | Yes | ||
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| Additional Information: | Funding Information: This work was financially supported by the Russian Science Foundation (project No. 19-14-00273) to YAK and an Australian Research Council (ARC) DECRA fellowship 180101563 to JJK. We thank Prof. Alexandr Nemec (Laboratory of Bacterial Genetics, National Institute of Public Health, Prague, Czech Republic) for supplying A. baumannii isolate NIPH 329. Computational resources and services used in this work were provided, in part, by the HPC and Research Support Group, Queensland University of Technology, Brisbane, Australia. | ||
| Measurements or Duration: | 13 pages | ||
| DOI: | 10.1371/journal.pone.0218461 | ||
| ISSN: | 1932-6203 | ||
| Pure ID: | 104906562 | ||
| Divisions: | Past > Institutes > Institute of Health and Biomedical Innovation | ||
| Funding Information: | This work was financially supported by the Russian Science Foundation (project No. 19-14-00273) to YAK and an Australian Research Council (ARC) DECRA fellowship 180101563 to JJK. We thank Prof. Alexandr Nemec (Laboratory of Bacterial Genetics, National Institute of Public Health, Prague, Czech Republic) for supplying A. baumannii isolate NIPH 329. Computational resources and services used in this work were provided, in part, by the HPC and Research Support Group, Queensland University of Technology, Brisbane, Australia. | ||
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| Copyright Owner: | 2019 The Author(s) | ||
| Copyright Statement: | This work is covered by copyright. Unless the document is being made available under a Creative Commons Licence, you must assume that re-use is limited to personal use and that permission from the copyright owner must be obtained for all other uses. If the document is available under a Creative Commons License (or other specified license) then refer to the Licence for details of permitted re-use. It is a condition of access that users recognise and abide by the legal requirements associated with these rights. If you believe that this work infringes copyright please provide details by email to qut.copyright@qut.edu.au | ||
| Deposited On: | 20 Jan 2022 05:33 | ||
| Last Modified: | 30 Mar 2024 17:45 |
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