An investigation of gene expression patterns that contribute to osmoregulation in Macrobrachium australiense: Assessment of adaptive responses to different osmotic niches
Description
<p>Osmoregulation is considered to be the principal mechanisms for survival and adaptation to different environmental salinity conditions. The present study was conducted to assess mRNA expression levels of five osmoregulatory genes in a Palaemonid prawn species, Macrobrachium australiense, exposed to three different salinities (0‰ 5‰ and 10‰) across six time intervals for a period of five days, using an RT-qPCR approach. Relative mRNA expression patterns revealed comparatively higher expression levels of Na<sup>+</sup>/K<sup>+</sup>-ATPase (NKA) and Na<sup>+</sup>/K<sup>+</sup>/2Cl<sup>−</sup> Co-transporter (NKCC) genes at raised salinities (5‰ and 10‰). At 0‰ vs 10‰ expression levels of these two genes were significantly higher (p < 0.05) and showed 2–3 fold and 4–6 fold higher (higher in 10‰ than 0‰) expression levels for NKA and NKCC, respectively. Significantly higher expression levels (p < 0.05) were observed at 0‰ for the Na<sup>+</sup>/H<sup>+</sup> exchanger (NHE) and V-type H<sup>+</sup>-ATPase (VTA) genes over 5‰ and 10‰ throughout the experimental time period. But initially higher expression levels of VTA were observed at 5‰ and 10‰ up to 24 h and then dropped below the levels at 0‰. The water channel regulatory gene, Aquaporin (AQP) showed very high expression levels initially (1 h) that were significantly different among treatments (p < 0.05), expression levels then declined up to the end of the experiment. In general, AQP, NHE and VTA were up-regulated in freshwater while NKA and NKCC were up-regulated under raised salinities. Results of this study indicate that the genes examined here likely play key roles in adaptive responses to variable environmental salinity conditions broadly in aquatic crustaceans.</p>
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| ID Code: | 228790 | ||
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| Item Type: | Contribution to Journal (Journal Article) | ||
| Refereed: | Yes | ||
| ORCID iD: |
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| Additional Information: | Funding Information: We gratefully acknowledge the comments and advice from the anonymous reviewer(s) that have significantly improved this manuscript. This study was funded by a QUT ( Queensland University of Technology ) Higher Degree Research (HDR) tuition sponsorship to the first author AM. We are grateful to MGRF staff (Vincent Chand, Kevin Dudley and Sahana Manoli) at QUT for their technical assistance. We would also like to thank all members of the Macrobrachium Genetics and Genomics Research group (Shengjie Ren and Dania Aziz) for assistance in the field and for valuable input and comments on this manuscript. | ||
| Measurements or Duration: | 8 pages | ||
| Keywords: | Gene expression, M. australiense, Osmoregulation, Palaemonid prawn, RT-qPCR | ||
| DOI: | 10.1016/j.genrep.2018.09.002 | ||
| ISSN: | 2452-0144 | ||
| Pure ID: | 106699657 | ||
| Divisions: | Past > QUT Faculties & Divisions > Science & Engineering Faculty | ||
| Funding Information: | We gratefully acknowledge the comments and advice from the anonymous reviewer(s) that have significantly improved this manuscript. This study was funded by a QUT ( Queensland University of Technology ) Higher Degree Research (HDR) tuition sponsorship to the first author AM. We are grateful to MGRF staff (Vincent Chand, Kevin Dudley and Sahana Manoli) at QUT for their technical assistance. We would also like to thank all members of the Macrobrachium Genetics and Genomics Research group (Shengjie Ren and Dania Aziz) for assistance in the field and for valuable input and comments on this manuscript. | ||
| Copyright Owner: | 2018 Elsevier Inc. | ||
| Copyright Statement: | This work is covered by copyright. Unless the document is being made available under a Creative Commons Licence, you must assume that re-use is limited to personal use and that permission from the copyright owner must be obtained for all other uses. If the document is available under a Creative Commons License (or other specified license) then refer to the Licence for details of permitted re-use. It is a condition of access that users recognise and abide by the legal requirements associated with these rights. If you believe that this work infringes copyright please provide details by email to qut.copyright@qut.edu.au | ||
| Deposited On: | 09 Mar 2022 05:17 | ||
| Last Modified: | 12 Jul 2024 07:08 |
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