Bactericidal Efficacy of Nanostructured Surfaces Increases under Flow Conditions
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Description
Bacterial colonization on solid surfaces creates enormous problems across various industries causing billions of dollars' worth of economic damages and costing human lives. Biomimicking nanostructured surfaces have demonstrated a promising future in mitigating bacterial colonization and related issues. The importance of this non-chemical method has been elevated due to bacterial evolvement into antibiotic and antiseptic-resistant strains. However, bacterial attachment and viability on nanostructured surfaces under fluid flow conditions has not been investigated thoroughly. In this study, attachment and viability of Pseudomonas aeruginosa (P. aeruginosa) and Staphylococcus aureus (S. aureus) on a model nanostructured surface were studied under fluid flow conditions. A wide range of flow rates resulting in a broad spectrum of fluid wall shear stress on a nanostructured surface representing various application conditions were experimentally investigated. The bacterial suspension was pumped through a custom-designed microfluidic device (MFD) that contains a sterile Ti-6Al-4V substrate. The surface of the titanium substrate was modified using a hydrothermal synthesis process to fabricate the nanowire structure on the surface. The results of the current study show that the fluid flow significantly reduces bacterial adhesion onto nanostructured surfaces and significantly reduces the viability of adherent cells. Interestingly, the bactericidal efficacy of the nanostructured surface was increased under the flow by 1.5-fold against P. aeruginosa and 3-fold against S. aureus under static conditions. The bactericidal efficacy had no dependency on the fluid wall shear stress level. However, trends in the dead-cell count with the fluid wall shear were slightly different between the two species. These findings will be highly useful in developing and optimizing nanostructures in the laboratory as well as translating them into successful industrial applications. These findings may be used to develop antibacterial surfaces on biomedical equipment such as catheters and vascular stents or industrial applications such as ship hulls and pipelines where bacterial colonization is a great challenge.
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ID Code: | 236733 | ||||||||
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Item Type: | Contribution to Journal (Journal Article) | ||||||||
Refereed: | Yes | ||||||||
ORCID iD: |
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Additional Information: | Acknowledgments: The authors wish to acknowledge support from the following individuals and institutions: Centre for Biomedical Technologies (CBT), Queensland University of Technology (QUT), Australia; Dr. K.A.S.N. Gunarathne for support in designing experiments with microbes; and Dr. Christina Theodoropoulos for support in fluorescence microscopy. Funding was received for this work from the AHEAD project (grant no. AHEAD/PhD/R2/ENG/TECH/161), University Grants Commission of Sri Lanka, and Queensland University of Technology. | ||||||||
Measurements or Duration: | 12 pages | ||||||||
DOI: | 10.1021/acsomega.2c05828 | ||||||||
ISSN: | 2470-1343 | ||||||||
Pure ID: | 118377956 | ||||||||
Divisions: | Current > Research Centres > Centre for Biomedical Technologies Current > QUT Faculties and Divisions > Faculty of Engineering Current > Schools > School of Mechanical, Medical & Process Engineering |
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Funding Information: | The authors wish to acknowledge support from the following individuals and institutions: Centre for Biomedical Technologies (CBT), Queensland University of Technology (QUT), Australia; Dr. K.A.S.N. Gunarathne for support in designing experiments with microbes; and Dr. Christina Theodoropoulos for support in fluorescence microscopy. Funding was received for this work from the AHEAD project (grant no. AHEAD/PhD/R2/ENG/TECH/161), University Grants Commission of Sri Lanka, and Queensland University of Technology. | ||||||||
Copyright Owner: | 2022 The Authors | ||||||||
Copyright Statement: | This work is covered by copyright. Unless the document is being made available under a Creative Commons Licence, you must assume that re-use is limited to personal use and that permission from the copyright owner must be obtained for all other uses. If the document is available under a Creative Commons License (or other specified license) then refer to the Licence for details of permitted re-use. It is a condition of access that users recognise and abide by the legal requirements associated with these rights. If you believe that this work infringes copyright please provide details by email to qut.copyright@qut.edu.au | ||||||||
Deposited On: | 06 Dec 2022 05:09 | ||||||||
Last Modified: | 10 May 2024 17:45 |
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