In Vitro Activities of Oxazolidinone Antibiotics Alone and in Combination with C-TEMPO against Methicillin-Resistant Staphylococcus aureus Biofilms
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Description
Infections caused by methicillin-resistant Staphylococcus aureus (MRSA) are a global health concern. The propensity of MRSA to form biofilms is a significant contributor to its pathogenicity. Strategies to treat biofilms often involve small molecules that disperse the biofilm into planktonic cells. Linezolid and, by extension, theoxazolidinones have been developed to treat infections caused by Gram-positive bacteria such as MRSA. However, the clinical development of these antibiotics has mainly assessed the susceptibility of planktonic cells to the drug. Previous studies evaluating the anti-biofilm activity of theoxazolidinones have mainly focused on the biofilm inhibition of Enterococcus faecalis and methicillin-sensitive Staphylococcus aureus, with only a few studies investigating the activity of oxazolidinones for eradicating established biofilms for these species. Very little is known about the ability of oxazolidinones to eradicate MRSA biofilms. In this work, five oxazolidinones were assessed against MRSA biofilms using a minimum biofilm eradication concentration (MBEC) assay. All oxazolidinones had inherent antibiofilm activity. However, only ranbezolid could completely eradicate MRSA biofilms at clinically relevant concentrations. The susceptibility of the MRSA biofilms to ranbezolid was synergistically enhanced by coadministration with the nitroxide biofilm dispersal agent C-TEMPO. We presume that ranbezolid acts as a dual warhead drug, which combines the mechanism of action of the oxazolidinones with a nitric oxide donor or cytotoxic drug.
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ID Code: | 245832 | ||||||||||
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Item Type: | Contribution to Journal (Journal Article) | ||||||||||
Refereed: | Yes | ||||||||||
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Additional Information: | Funding Information: This research was funded by the Australian Research Council’s Future Fellowship, FT140100746 to K.E.F.-S. and Discovery Project, DP210101317 to K.E.F.-S. and M.T. A.N. is supported by an Australian Government Research Training Program (RTP) Scholarship. M.T. acknowledges support from the Max Planck Queensland Centre on the Materials Science of Extracellular Matrices. | ||||||||||
Measurements or Duration: | 14 pages | ||||||||||
Keywords: | antibiofilm, antimicrobial, biofilm, biofilm eradication, MBEC, MRSA, nitroxide, oxazolidinone, Staphylococcus aureus | ||||||||||
DOI: | 10.3390/antibiotics12121706 | ||||||||||
ISSN: | 2079-6382 | ||||||||||
Pure ID: | 156155923 | ||||||||||
Divisions: | Current > Research Centres > Centre for Materials Science Current > Research Centres > Centre for Immunology and Infection Control Current > QUT Faculties and Divisions > Faculty of Science Current > Schools > School of Chemistry & Physics Current > QUT Faculties and Divisions > Faculty of Health Current > Schools > School of Biomedical Sciences |
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Funding Information: | We gratefully acknowledge financial support for this work from the Ian Potter Foundation sponsorship of specialist equipment at QUT’s Centre for Immunology and Infection Control. We also acknowledge the Centre for Materials Science and facilities of QUT’s Central Analytical Research Facility (CARF), which is supported by funding from the Faculty of Science (QUT). This research was funded by the Australian Research Council’s Future Fellowship, FT140100746 to K.E.F.-S. and Discovery Project, DP210101317 to K.E.F.-S. and M.T. A.N. is supported by an Australian Government Research Training Program (RTP) Scholarship. M.T. acknowledges support from the Max Planck Queensland Centre on the Materials Science of Extracellular Matrices. | ||||||||||
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Copyright Owner: | 2023 The Authors | ||||||||||
Copyright Statement: | This work is covered by copyright. Unless the document is being made available under a Creative Commons Licence, you must assume that re-use is limited to personal use and that permission from the copyright owner must be obtained for all other uses. If the document is available under a Creative Commons License (or other specified license) then refer to the Licence for details of permitted re-use. It is a condition of access that users recognise and abide by the legal requirements associated with these rights. If you believe that this work infringes copyright please provide details by email to qut.copyright@qut.edu.au | ||||||||||
Deposited On: | 24 Jan 2024 05:25 | ||||||||||
Last Modified: | 23 Apr 2024 16:01 |
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