Whole-mount in situ hybridization in Amphimedon
Description
Developmental gene expression is analyzed predominantly via whole-mount in situ hybridization using digoxigenin-labeled RNA probes. This protocol describes how to perform this procedure in Amphimedon queenslandica, including fixation, hybridization, and sectioning of embryonic, larval, and post-larval juvenile stages.
Impact and interest:
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| ID Code: | 248692 | ||
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| Item Type: | Contribution to Journal (Journal Article) | ||
| Refereed: | Yes | ||
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| Measurements or Duration: | 8 pages | ||
| DOI: | 10.1101/pdb.prot5096 | ||
| ISSN: | 1559-6095 | ||
| Pure ID: | 169307699 | ||
| Funding Information: | This research has been supported by grants from the Australian Research Council. | ||
| Copyright Owner: | 2008 Cold Spring Harbor Laboratory Press | ||
| Copyright Statement: | This work is covered by copyright. Unless the document is being made available under a Creative Commons Licence, you must assume that re-use is limited to personal use and that permission from the copyright owner must be obtained for all other uses. If the document is available under a Creative Commons License (or other specified license) then refer to the Licence for details of permitted re-use. It is a condition of access that users recognise and abide by the legal requirements associated with these rights. If you believe that this work infringes copyright please provide details by email to qut.copyright@qut.edu.au | ||
| Deposited On: | 24 May 2024 01:17 | ||
| Last Modified: | 24 May 2024 21:08 |
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