Isolation of Amphimedon developmental material
Description
Fertilization occurs internally in Amphimedon and embryos are brooded in multiple chambers throughout the adult. Each chamber contains a mixture of developmental stages, from egg to late ring stages (i.e., prehatch late embryos). At the end of embryogenesis, swimming parenchymella larvae emerge from the adult. After several hours in the water column, the larvae settle and metamorphose into juvenile sponges. This protocol details how to obtain Amphimedon larvae and post-larvae/juveniles as well as embryos. Once isolated, these biological stages can be used for a variety of molecular and cellular analyses.
Impact and interest:
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| ID Code: | 248693 | ||
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| Item Type: | Contribution to Journal (Journal Article) | ||
| Refereed: | Yes | ||
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| Measurements or Duration: | 5 pages | ||
| DOI: | 10.1101/pdb.prot5095 | ||
| ISSN: | 1559-6095 | ||
| Pure ID: | 169308156 | ||
| Funding Information: | This research has been supported by grants from the Australian Research Council. | ||
| Copyright Owner: | 2008 Cold Spring Harbor Laboratory Press | ||
| Copyright Statement: | This work is covered by copyright. Unless the document is being made available under a Creative Commons Licence, you must assume that re-use is limited to personal use and that permission from the copyright owner must be obtained for all other uses. If the document is available under a Creative Commons License (or other specified license) then refer to the Licence for details of permitted re-use. It is a condition of access that users recognise and abide by the legal requirements associated with these rights. If you believe that this work infringes copyright please provide details by email to qut.copyright@qut.edu.au | ||
| Deposited On: | 24 May 2024 01:19 | ||
| Last Modified: | 24 May 2024 21:08 |
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