[n]Cycloparaphenylenes as Compatible Fluorophores for Melt Electrowriting
Description
Fluorescent probes are an indispensable tool in the realm of bioimaging technologies, providing valuable insights into the assessment of biomaterial integrity and structural properties. However, incorporating fluorophores into scaffolds made from melt electrowriting (MEW) poses a challenge due to the sustained, elevated temperatures that this processing technique requires. In this context, [n]cycloparaphenylenes ([n]CPPs) serve as excellent fluorophores for MEW processing with the additional benefit of customizable emissions profiles with the same excitation wavelength. Three fluorescent blends are used with distinct [n]CPPs with emission wavelengths of either 466, 494, or 533 nm, identifying 0.01 wt% as the preferred concentration. It is discovered that [n]CPPs disperse well within poly(ε-caprolactone) (PCL) and maintain their fluorescence even after a week of continuous heating at 80 °C. The [n]CPP-PCL blends show no cytotoxicity and support counterstaining with commonly used DAPI (Ex/Em: 359 nm/457 nm), rhodamine- (Ex/Em: 542/565 nm), and fluorescein-tagged (Ex/Em: 490/515 nm) phalloidin stains. Using different color [n]CPP-PCL blends, different MEW fibers are sequentially deposited into a semi-woven scaffold and onto a solution electrospun membrane composed of [8]CPP-PCL as a contrasting substrate for the [10]CPP-PCL MEW fibers. In general, [n]CPPs are potent fluorophores for MEW, providing new imaging options for this technology.
Impact and interest:
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ID Code: | 249033 | ||
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Item Type: | Contribution to Journal (Journal Article) | ||
Refereed: | Yes | ||
ORCID iD: |
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Measurements or Duration: | 11 pages | ||
Keywords: | additive manufacturing, biofabrication, electrospinning, scaffolds, tissue engineering | ||
DOI: | 10.1002/smll.202400882 | ||
ISSN: | 1613-6810 | ||
Pure ID: | 171200027 | ||
Divisions: | Current > Research Centres > Centre for Biomedical Technologies Current > QUT Faculties and Divisions > Faculty of Engineering Current > Schools > School of Mechanical, Medical & Process Engineering |
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Funding Information: | The financial support from the Wu Tsai Human Performance Alliance and the Joe and Clara Tsai Foundation is greatly appreciated. P.D. was supported by the Bradshaw and Holzapfel Research Professor in Transformational Science and Mathematics Fund. H.W.R. and R.J. were supported by the U.S. National Science Foundation (CHE-2102567). | ||
Copyright Owner: | 2024 Wiley-VCH GmbH. | ||
Copyright Statement: | This work is covered by copyright. Unless the document is being made available under a Creative Commons Licence, you must assume that re-use is limited to personal use and that permission from the copyright owner must be obtained for all other uses. If the document is available under a Creative Commons License (or other specified license) then refer to the Licence for details of permitted re-use. It is a condition of access that users recognise and abide by the legal requirements associated with these rights. If you believe that this work infringes copyright please provide details by email to qut.copyright@qut.edu.au | ||
Deposited On: | 14 Jun 2024 03:21 | ||
Last Modified: | 16 Jun 2024 22:10 |
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