Inactivation of Viruses in Bubbling Processes Utilized for Personal Bioaerosol Monitoring

Agranovski, Igor E., Safatov, Alexander S., Borodulin, Alexander I., Pyankov, Oleg V., Petrishchenko, Valentina A., Sergeev, Alexander N., Agafanov, Alexander, Ignatiev, Georgy, Sergeev, Artemii, & Agranovski, Victoria (2004) Inactivation of Viruses in Bubbling Processes Utilized for Personal Bioaerosol Monitoring. Applied and Environmental Microbiology, 70(12), pp. 6963-6967.

View at publisher (open access)


A new personal bioaerosol sampler has recently been developed and evaluated for sampling of viable airborne bacteria and fungi under controlled laboratory conditions and in the field. The operational principle of the device is based on the passage of air through porous medium immersed in liquid. This process leads to the formation of bubbles within the filter as the carrier gas passes through and thus provides effective mechanisms for aerosol removal. As demonstrated in previous studies, the culturability of sampled bacterium and fungi remained high for the entire 8-h sampling period. The present study is the first step of the evaluation of the new sampler for monitoring of viable airborne viruses. It focuses on the investigation of the inactivation rate of viruses in the bubbling process during 4 h of continuous operation. Four microbes were used in this study, influenza, measles, mumps, and vaccinia viruses. It was found that the use of distilled water as the collection fluid was associated with a relatively high decay rate. A significant improvement was achieved by utilizing virus maintenance fluid prepared by using Hank’s solution with appropriate additives. The survival rates of the influenza, measles, and mumps viruses were increased by 1.4 log, 0.83 log, and 0.82 log, respectively, after the first hour of operation compared to bubbling through the sterile water. The same trend was observed throughout the entire 4-h experiment. There was no significant difference observed only for the robust vaccinia virus.

Impact and interest:

40 citations in Scopus
39 citations in Web of Science®
Search Google Scholar™

Citation counts are sourced monthly from Scopus and Web of Science® citation databases.

These databases contain citations from different subsets of available publications and different time periods and thus the citation count from each is usually different. Some works are not in either database and no count is displayed. Scopus includes citations from articles published in 1996 onwards, and Web of Science® generally from 1980 onwards.

Citations counts from the Google Scholar™ indexing service can be viewed at the linked Google Scholar™ search.

ID Code: 5790
Item Type: Journal Article
Refereed: Yes
Additional Information: Articles free to read on journal website after 6 months
DOI: 10.1128/AEM.70.12.6963-6967.2004
ISSN: 0099-2240
Subjects: Australian and New Zealand Standard Research Classification > EARTH SCIENCES (040000) > ATMOSPHERIC SCIENCES (040100) > Atmospheric Sciences not elsewhere classified (040199)
Divisions: Past > QUT Faculties & Divisions > Faculty of Science and Technology
Copyright Owner: Copyright 2004 American Society for Microbiology
Deposited On: 20 Dec 2006 00:00
Last Modified: 21 Mar 2016 05:22

Export: EndNote | Dublin Core | BibTeX

Repository Staff Only: item control page