Establishment and characterisation of an open mini- thoracotomy surgical approach to an ovine thoracic spine fusion model
Yong, Mostyn, Saifzadeh, Siamak, Askin, Geoffrey, Labrom, Robert, Hutmacher, Dietmar, & Adam, Clayton (2014) Establishment and characterisation of an open mini- thoracotomy surgical approach to an ovine thoracic spine fusion model. Tissue Engineering, Part C: Methods, 20(1), pp. 19-27.
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Description
Background A large animal model is required for assessment of minimally invasive, tissue engineering based approaches to thoracic spine fusion, with relevance to deformity correction surgery for human adolescent idiopathic scoliosis. Here we develop a novel open mini–thoracotomy approach in an ovine model of thoracic interbody fusion which allows assessment of various fusion constructs, with a focus on novel, tissue engineering based interventions. Methods The open mini-thoracotomy surgical approach was developed through a series of mock surgeries, and then applied in a live sheep study. Customized scaffolds were manufactured to conform with intervertebral disc space clearances required of the study. Twelve male Merino sheep aged 4 to 6 years and weighing 35 – 45 kg underwent the abovementioned procedure and were divided into two groups of six sheep at survival timelines of 6 and 12 months. Each sheep underwent a 3-level discectomy (T6/7, T8/9 and T10/11) with randomly allocated implantation of a different graft substitute at each of the three levels; (i) polycaprolactone (PCL) based scaffold plus 0.54μg rhBMP-2, (ii) PCL-based scaffold alone or (iii) autograft. The sheep were closely monitored post- operatively for signs of pain (i.e. gait abnormalities/ teeth gnawing/ social isolation). Fusion assessments were conducted post-sacrifice using Computed Tomography and hard-tissue histology. All scientific work was undertaken in accordance with the study protocol has been approved by the Institute's committee on animal research. Results. All twelve sheep were successfully operated on and reached the allotted survival timelines, thereby demonstrating the feasibility of the surgical procedure and post-operative care. There were no significant complications and during the post-operative period the animals did not exhibit marked signs of distress according to the described assessment criteria. Computed Tomographic scanning demonstrated higher fusion grades in the rhBMP-2 plus PCL-based scaffold group in comparison to either PCL-based scaffold alone or autograft. These results were supported by histological evaluation of the respective groups. Conclusion. This novel open mini-thoracotomy surgical approach to the ovine thoracic spine represents a safe surgical method which can reproducibly form the platform for research into various spine tissue engineered constructs (TEC) and their fusion promoting properties.
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ID Code: | 60840 | ||||||||
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Item Type: | Contribution to Journal (Journal Article) | ||||||||
Refereed: | Yes | ||||||||
ORCID iD: |
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Measurements or Duration: | 9 pages | ||||||||
Keywords: | bone regeneration, growth factors, open mini-thoracotomy, polycaprolactone, preclinical animal model, sheep thoracic spine, spinal surgery | ||||||||
DOI: | 10.1089/ten.TEC.2012.0746 | ||||||||
ISSN: | 1937-3392 | ||||||||
Pure ID: | 32668037 | ||||||||
Divisions: | Past > Institutes > Institute of Health and Biomedical Innovation Past > QUT Faculties & Divisions > Science & Engineering Faculty |
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Copyright Owner: | Consult author(s) regarding copyright matters | ||||||||
Copyright Statement: | This work is covered by copyright. Unless the document is being made available under a Creative Commons Licence, you must assume that re-use is limited to personal use and that permission from the copyright owner must be obtained for all other uses. If the document is available under a Creative Commons License (or other specified license) then refer to the Licence for details of permitted re-use. It is a condition of access that users recognise and abide by the legal requirements associated with these rights. If you believe that this work infringes copyright please provide details by email to qut.copyright@qut.edu.au | ||||||||
Deposited On: | 19 Jun 2013 00:42 | ||||||||
Last Modified: | 10 Feb 2025 09:42 |
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