Expression of mineralization markers in dental pulp cells

Wei, Xi, Ling, Junqi, Wu, Liping, Liu, Lu, & Xiao, Yin (2007) Expression of mineralization markers in dental pulp cells. Journal of Endodontics, 33(6), pp. 703-708.

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There is an increasing interest in the utility of dental pulp stem cells (DPSCs) for dentin regeneration. The mechanisms involved in DPSC differentiation remain poorly understood. The purpose of the study was to investigate the mineralization capacity of human dental pulp cells (DPCs) and identify potential markers for odontoblast differentiation. The isolated DPCs expressed mesenchymal stem cell markers as shown by flow cytometry and could differentiate in vitro into odontogenic, adipogenic and chondrogenic lineages. Alkaline phosphatase activity of DPCs elevated over time, with significant upregulation on day 21 in odontogenic induction. Quantitative RT-PCR revealed that osteocalcin, dentin sialophosphoprotein (DSPP) and matrix extracellular phosphoglycoprotein (MEPE) expression also increased time-dependently in the induction cultures. In conclusion, we isolated DPCs with stem cell characteristics. DSPP and MEPE expression increased in an analogous way with cellular progression along odontoblast lineage. MEPE along with DSPP may be potential odontogenetic differentiation markers.

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92 citations in Web of Science®
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ID Code: 6319
Item Type: Journal Article
Refereed: Yes
Additional Information: For more information, please refer to the journal’s website (see link) or contact the author. Author contact details:
Keywords: Dental pulp stem cells (DPSCs), odontoblast, differentiation, dentin sialophosphoprotein (DSPP), matrix extracellular phosphoglycoprotein (MEPE)
DOI: 10.1016/j.joen.2007.02.009
ISSN: 0099-2399
Divisions: Past > QUT Faculties & Divisions > Faculty of Built Environment and Engineering
Copyright Owner: Copyright 2007 Elsevier
Deposited On: 03 May 2007 00:00
Last Modified: 16 Apr 2015 06:17

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