Functional hepatocyte cation compartmentation demonstrated with 133Cs NMR
Description
This study utilized the large intrinsic chemical shift range of 133Cs, a potassium congener, in an NMR study of intracellular cation distribution. It demonstrates two distinct intracellular environments in isolated perfused hepatocytes from cesiumfed rats, evident as compartments with different 133Cs chemical shifts and containing different proportions of total detected cesium. The chemical shifts of the two intracellular compartments were 2.44 0.07 and 1.21 0.18 ppm, relative to the cesium signal from the perfusate. The observation of two distinct intracellular cesium signals suggests slow exchange on an NMR chemical shift time-scale (k exchange > 0.02 s). The area of the high-frequency component represented 62 10% (N 12) of the total intracellular cesium signal. Manipulation of the intracellular environment using anoxia with aglycemia or digitonin produced changes in the distribution between the two intracellular compartments, showing their dynamic nature. Changes measured in association with metabolic manipulation suggest cytoplasm and mitochondria as the origin of the high and low-frequency intracellular peaks, respectively.
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ID Code: | 6848 | ||
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Item Type: | Contribution to Journal (Journal Article) | ||
Refereed: | Yes | ||
ORCID iD: |
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Measurements or Duration: | 9 pages | ||
DOI: | 10.1002/mrm.10287 | ||
ISSN: | 0740-3194 | ||
Pure ID: | 34073832 | ||
Divisions: | Past > QUT Faculties & Divisions > Faculty of Science and Technology | ||
Copyright Owner: | Consult author(s) regarding copyright matters | ||
Copyright Statement: | This work is covered by copyright. Unless the document is being made available under a Creative Commons Licence, you must assume that re-use is limited to personal use and that permission from the copyright owner must be obtained for all other uses. If the document is available under a Creative Commons License (or other specified license) then refer to the Licence for details of permitted re-use. It is a condition of access that users recognise and abide by the legal requirements associated with these rights. If you believe that this work infringes copyright please provide details by email to qut.copyright@qut.edu.au | ||
Deposited On: | 28 Mar 2007 00:00 | ||
Last Modified: | 03 Mar 2024 16:23 |
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