Structure of the K2 capsule associated with the KL2 gene cluster of Acinetobacter baumannii

, Marzaioli, Alberto, Hall, Ruth, & De Castro, Cristina (2014) Structure of the K2 capsule associated with the KL2 gene cluster of Acinetobacter baumannii. Glycobiology, 24(6), pp. 554-563.

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Description

The repeat unit structure of the K2 capsule from an extensively antibiotic-resistant Acinetobacter baumannii global clone 2 (GC2) strain was determined. The oligosaccharide contains three simple sugars, d-glucopyranose, d-galatopyranose and N-acetyl-d-galactosamine, and the complex sugar, 5,7-diacetamido-3,5,7,9-tetradeoxy-l-glycero-l-manno-non-2-ulosonic acid (Pse5Ac7Ac or pseudaminic acid), which has not previously been reported in any A. baumannii capsule. The strain was found to carry all the genes required for the synthesis of the sugars and construction of the K2 structure. The linkages catalyzed by the initiating transferase, three glycosyltransferases and the Wzy polymerase were also predicted. Examination of publicly available A. baumannii genome sequences revealed that the same gene cluster, KL2, often occurs in extensively antibiotic-resistant GC2 isolates and in further strain types. The gene module responsible for the synthesis of pseudaminic acid was also detected in four other K loci. A related module including genes for an acylated relative of pseudaminic acid was also found in two new KL types. A polymerase chain reaction scheme was developed to detect all modules containing genes for sugars based on pseudaminic acid and to specifically detect KL2.

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ID Code: 83183
Item Type: Contribution to Journal (Journal Article)
Refereed: Yes
ORCID iD:
Kenyon, Johannaorcid.org/0000-0002-1487-6105
Measurements or Duration: 10 pages
Keywords: Acinetobacter baumannii, Genetic variation, K locus, bacterial pathogen, capsule, clone, hospital-acquired infection, polysaccharide, pseudaminic acid
DOI: 10.1093/glycob/cwu024
ISSN: 1460-2423
Pure ID: 32754082
Divisions: Past > QUT Faculties & Divisions > Faculty of Health
Past > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: Consult author(s) regarding copyright matters
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Deposited On: 02 Dec 2015 22:31
Last Modified: 07 May 2024 22:28