Effects of hyperbaric oxygen on proliferation and differentiation of osteoblasts derived from human alveolar bone

Wu, Dong, Malda, Jos, Crawford, Ross W., & Xiao, Yin (2007) Effects of hyperbaric oxygen on proliferation and differentiation of osteoblasts derived from human alveolar bone. Connective Tissue Research, 48(4), pp. 206-213.

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In view of the controversy of the clinical use of hyperbaric oxygen (HBO) treatment to stimulate fracture healing and bone regeneration, we have analysed the effects of daily exposure to HBO on the proliferation and differentiation of human osteoblasts in vitro. HBO stimulated proliferation when osteoblasts were cultured in 10% foetal calf serum (FCS), whereas an inhibitory effect of HBO was observed when cultures were supplemented with 2% FCS,. On the other hand, HBO enhanced biomineralization with an increase in bone nodule formation, calcium deposition and alkaline phosphatase activity, while no cytotoxic effect was detected using a lactate dehydrogenase activity assay. The data suggests that the exposure of osteoblasts to HBO enhances differentiation towards the osteogenic phenotype, providing cellular evidence of the potential application of HBO in fracture healing and bone regeneration.

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36 citations in Scopus
30 citations in Web of Science®
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ID Code: 8583
Item Type: Journal Article
Refereed: Yes
DOI: 10.1080/03008200701458749
ISSN: 0300-8207
Subjects: Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > BIOCHEMISTRY AND CELL BIOLOGY (060100) > Cell Development Proliferation and Death (060103)
Divisions: Past > QUT Faculties & Divisions > Faculty of Built Environment and Engineering
Current > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: Copyright 2007 Taylor & Francis
Copyright Statement: First published in Connective Tissue Research 48(4):pp. 206-213.
Deposited On: 12 Jul 2007 00:00
Last Modified: 29 Feb 2012 13:31

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