Surplus zinc is handled by Zym1 Metallothionein and Zhf endoplasmic reticulum transporter in schizosaccharomyces pombe

Borrelly, Gilles P. M., Harrison, Mark D., Robinson, Andrea K., Cox, Samuel G., Robinson, Nigel J., & Whitehall, Simon K. (2002) Surplus zinc is handled by Zym1 Metallothionein and Zhf endoplasmic reticulum transporter in schizosaccharomyces pombe. Journal of Biological Chemistry, 277(33), pp. 30394-30400.

This is the latest version of this eprint.

View at publisher (open access)


Homeostatic mechanisms prevent the accumulation of free zinc in the cytoplasm, raising questions regarding where surplus zinc is stored and how it is delivered to and from these stores. A genetic screen for zinc hypersensitivity in Schizosaccharomyces pombe identified a missense mutation truncating Zhf, an endoplasmic reticulum transporter. These cells were ~5-fold more zinc-sensitive than other independent mutants. The targeted disruption of zhf prevented growth on low zinc medium and caused hypersensitivity to elevated zinc/cobalt but resistance to cadmium. The exposure to elevated zinc but not copper also promotes the accumulation of transcripts encoding a metallothionein designated Zym1. The Sty1 pathway is required for maximal zym1 expression but is not obligatory for zinc perception. The targeted disruption of zym1 impaired cadmium tolerance but only slightly impaired zinc tolerance, whereas zym1 overexpression substantially rescued zinc hypersensitivity of zhf cells. Four equivalents of zinc were displaced from Zym1 by up to 12 equivalents of p-(hydroxymercuri)phenylsulphonate. Zym1 thiols react rapidly with 5,5'-dithiobis-(2-nitrobenzoic acid) compared with bacterial zinc metallothionein (6.8 and 0.2 × 104 s1, respectively). Zym1 is unlike known fungal metallothioneins that are induced by and sequester copper but not zinc. Less zinc but normal cadmium was accumulated by zym1, consistent with zinc sequestration by Zym1 in vivo.

Impact and interest:

48 citations in Scopus
45 citations in Web of Science®
Search Google Scholar™

Citation counts are sourced monthly from Scopus and Web of Science® citation databases.

These databases contain citations from different subsets of available publications and different time periods and thus the citation count from each is usually different. Some works are not in either database and no count is displayed. Scopus includes citations from articles published in 1996 onwards, and Web of Science® generally from 1980 onwards.

Citations counts from the Google Scholar™ indexing service can be viewed at the linked Google Scholar™ search.

Full-text downloads:

2 since deposited on 14 Aug 2007
2 in the past twelve months

Full-text downloads displays the total number of times this work’s files (e.g., a PDF) have been downloaded from QUT ePrints as well as the number of downloads in the previous 365 days. The count includes downloads for all files if a work has more than one.

ID Code: 8970
Item Type: Journal Article
Refereed: Yes
Additional Information: This research was originally published in the Journal of Biological Chemistry. Gilles P. M. Borrelly, Mark D. Harrison, Andrea K. Robinson, Samuel G. Cox, Nigel J. Robinson and Simon K. Whitehall. Surplus Zinc Is Handled by Zym1 Metallothionein and Zhf Endoplasmic Reticulum Transporter in Schizosaccharomyces pombe. Journal of Biological Chemistry. 2002; 277:30394-30400. © the American Society for Biochemistry and Molecular Biology.
Additional URLs:
DOI: 10.1074/jbc.M203145200
ISSN: 0021-9258
Divisions: Past > QUT Faculties & Divisions > Faculty of Science and Technology
Copyright Owner: Copyright 2002 The American Society for Biochemistry and Molecular Biology
Deposited On: 14 Aug 2007 00:00
Last Modified: 15 May 2017 00:02

Available Versions of this Item

  • Surplus zinc is handled by Zym1 Metallothionein and Zhf endoplasmic reticulum transporter in schizosaccharomyces pombe. (deposited 14 Aug 2007 00:00) [Currently Displayed]

Export: EndNote | Dublin Core | BibTeX

Repository Staff Only: item control page