Scaffold-guided bone regeneration in large volume tibial segmental defects
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101962712. Available under License Creative Commons Attribution Non-commercial No Derivatives 2.5. |
Description
Large volume losses in weight bearing long bones are a major challenge in clinical practice. Despite multiple innovations over the last decades, significant limitations subsist in current clinical treatment options which is driving a strong clinical demand for clinically translatable treatment alternatives, including bone tissue engineering applications. Despite these shortcomings, preclinical large animal models of large volume segmental bone defects to investigate the regenerative capacity of bone tissue engineering strategies under clinically relevant conditions are rarely described in literature. We herein present a newly established preclinical ovine animal model for the treatment of XL volume (19 cm3) segmental tibial defects. In eight aged male Merino sheep (age > 6 years) a mid-diaphyseal tibial segmental defect was created and stabilized with a 5.6 mm Dynamic Compression Plate (DCP). We present short-term (3 months) and long-term (12–15 months) results of a pilot study using medical grade Polycaprolactone-Tricalciumphosphate (mPCL-TCP) scaffolds combined with a dose of 2 mg rhBMP-7 delivered in Platelet-Rich- Plasma (PRP). Furthermore, detailed analyses of the mechanical properties of the scaffolds as well as interfragmentary movement (IFM) and DCP-surface strain in vitro and a comprehensive description of the surgical and post-surgery protocol and post-mortem analysis is given.
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ID Code: | 226458 | ||||||||||||||
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Item Type: | Contribution to Journal (Journal Article) | ||||||||||||||
Refereed: | Yes | ||||||||||||||
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Additional Information: | Funding Information: We would like to thank the staff of the Queensland University of Technology Medical Engineering Research Facility (MERF) for assistance and administrative and technical support. We thank the Queensland University of Technology (QUT) Biofabrication and Tissue Morphology Group and QUT CARF Histology Facility for helping with the preparation of the histological specimen. We thank Cameron Black for helping with processing the histological samples. We also would like to thank Dr. med. Markus Laubach for editing the biomechanical testing image. This research was supported by The Australian Research Council Industrial Transformation Training Center in Additive Biomanufacturing [grant number IC160100026]; and the ARC Industrial Transformation Training Centre (ARC ITTC) for Multiscale 3D Imaging, Modelling and Manufacturing [grant number IC180100008]. A part of this work was supported by funding through the German Research Foundation [Grants BE 4492/1-2 and HE 7074/1-1]. | ||||||||||||||
Measurements or Duration: | 15 pages | ||||||||||||||
Keywords: | Bone morphogenetic protein, Bone tissue engineering, Large volume bone defect, Ovine, Polycaprolactone, Preclinical animal model, Scaffold, Sheep | ||||||||||||||
DOI: | 10.1016/j.bone.2021.116163 | ||||||||||||||
ISSN: | 8756-3282 | ||||||||||||||
Pure ID: | 101962712 | ||||||||||||||
Divisions: | Current > Research Centres > Centre for Behavioural Economics, Society & Technology Current > Research Centres > Centre for Biomedical Technologies Current > Research Centres > Centre for Transformative Biomimetics in Bioeng Current > Research Centres > Centre for Healthcare Transformation Current > QUT Faculties and Divisions > Academic Division Current > QUT Faculties and Divisions > Faculty of Business & Law Current > QUT Faculties and Divisions > Faculty of Engineering Current > Schools > School of Mechanical, Medical & Process Engineering Current > QUT Faculties and Divisions > Faculty of Health |
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Funding Information: | We would like to thank the staff of the Queensland University of Technology Medical Engineering Research Facility (MERF) for assistance and administrative and technical support. We thank the Queensland University of Technology (QUT) Biofabrication and Tissue Morphology Group and QUT CARF Histology Facility for helping with the preparation of the histological specimen. We thank Cameron Black for helping with processing the histological samples. We also would like to thank Dr. med. Markus Laubach for editing the biomechanical testing image. This research was supported by The Australian Research Council Industrial Transformation Training Center in Additive Biomanufacturing [grant number IC160100026]; and the ARC Industrial Transformation Training Centre (ARC ITTC) for Multiscale 3D Imaging, Modelling and Manufacturing [grant number IC180100008]. A part of this work was supported by funding through the German Research Foundation [Grants BE 4492/1-2 and HE 7074/1-1]. This research was supported by The Australian Research Council Industrial Transformation Training Center in Additive Biomanufacturing [grant number IC160100026 ]; and the ARC Industrial Transformation Training Centre (ARC ITTC) for Multiscale 3D Imaging, Modelling and Manufacturing [grant number IC180100008 ]. A part of this work was supported by funding through the German Research Foundation [Grants BE 4492/1-2 and HE 7074/1-1 ]. | ||||||||||||||
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Copyright Owner: | Crown Copyright 2021. | ||||||||||||||
Copyright Statement: | This work is covered by copyright. Unless the document is being made available under a Creative Commons Licence, you must assume that re-use is limited to personal use and that permission from the copyright owner must be obtained for all other uses. If the document is available under a Creative Commons License (or other specified license) then refer to the Licence for details of permitted re-use. It is a condition of access that users recognise and abide by the legal requirements associated with these rights. If you believe that this work infringes copyright please provide details by email to qut.copyright@qut.edu.au | ||||||||||||||
Deposited On: | 25 Nov 2021 22:51 | ||||||||||||||
Last Modified: | 28 Jul 2024 12:00 |
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