ReX: A suite of computational tools for the design, visualization, and analysis of chimeric protein libraries
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114654960. Available under License Creative Commons Attribution 4.0. |
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Description
Directed evolution has greatly facilitated protein engineering and provided new insights into protein structure–function relationships. DNA shuffling using restriction enzymes is a particularly simple and cost-effective means of recombinatorial evolution that is well within the capability of most molecular biologists, but tools for the design and analysis of such experiments are limited. Here we introduce a suite of freely available online tools to make the construction and analysis of chimeric libraries readily accessible to the novice. REcut (http://qpmf.rx.umaryland.edu/REcut.html) facilitates the choice of DNA fragmentation strategy, while Xover (http://qpmf.rx.umaryland.edu/Xover.html) analyzes chimeric mutants to reveal recombination patterns and extract quantitative data.
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| ID Code: | 234871 | ||
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| Item Type: | Contribution to Journal (Journal Article) | ||
| Refereed: | Yes | ||
| ORCID iD: |
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| Measurements or Duration: | 4 pages | ||
| Keywords: | Artificial evolution, Chimera, Crossover, Directed evolution, DNA shuffling, Mosaic, Mutant library, PCR, Recombination, Restriction enzyme, SCHEMA, Staggered extension process | ||
| DOI: | 10.2144/000114381 | ||
| ISSN: | 0736-6205 | ||
| Pure ID: | 114654960 | ||
| Copyright Owner: | Consult author(s) regarding copyright matters | ||
| Copyright Statement: | This work is covered by copyright. Unless the document is being made available under a Creative Commons Licence, you must assume that re-use is limited to personal use and that permission from the copyright owner must be obtained for all other uses. If the document is available under a Creative Commons License (or other specified license) then refer to the Licence for details of permitted re-use. It is a condition of access that users recognise and abide by the legal requirements associated with these rights. If you believe that this work infringes copyright please provide details by email to qut.copyright@qut.edu.au | ||
| Deposited On: | 24 Aug 2022 02:17 | ||
| Last Modified: | 26 Jul 2024 21:29 |
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