Browse By Person: Johnston, Wayne

Alfaro-Palma, Juan, Johnston, Wayne A., Behrendorff, James, Cui, Zhenling, Moradi, Shayli Varasteh, & Alexandrov, Kirill (2024) Development of Lyophilized Eukaryotic Cell-Free Protein Expression System Based on Leishmania tarentolae. ACS Synthetic Biology, 13(2), pp. 449-456.

Navone, Laura, Moffitt, Kaylee, Johnston, Wayne A., Mercer, Tim, Cooper, Crystal, Spann, Kirsten, & Speight, Robert E. (2022) Bioengineered textiles with peptide binders that capture SARS-CoV-2 viral particles. Communications Materials, 3, Article number: 54.

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Guo, Zhong, Parakra, Rinky D., Xiong, Ying, Johnston, Wayne A., Walden, Patricia, Edwardraja, Selvakumar, Moradi, Shayli Varasteh, Ungerer, Jacobus P.J., Ai, Hui wang, Phillips, Jonathan J., & Alexandrov, Kirill (2022) Engineering and exploiting synthetic allostery of NanoLuc luciferase. Nature Communications, 13, Article number: 789.

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Ergun Ayva, Cagla, Fiorito, Maria M., Guo, Zhong, Edwardraja, Selvakumar, Kaczmarski, Joe A., Gagoski, Dejan, Walden, Patricia, Johnston, Wayne A., Jackson, Colin J., Nebl, Tom, & Alexandrov, Kirill (2022) Exploring Performance Parameters of Artificial Allosteric Protein Switches. Journal of Molecular Biology, 434(17), Article number: 167678.

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Ryan, Stephanie M., Ruscher, Roland, Johnston, Wayne A., Pickering, Darren A., Kennedy, Malcolm W., Smith, Brian O., Jones, Linda, Buitrago, Geraldine, Field, Matt A., Esterman, Adrian J., McHugh, Connor P., Browne, Daniel J., Cooper, Martha M., Ryan, Rachael Y.M., Doolan, Denise L., Engwerda, Christian R., Miles, Kim, Mitreva, Makedonka, Croese, John, Rahman, Tony, Alexandrov, Kirill, Giacomin, Paul R., & Loukas, Alex (2022) Novel antiinflammatory biologics shaped by parasite-host coevolution. Proceedings of the National Academy of Sciences of the United States of America, 119(36), Article number: e2202795119.

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Guo, Zhong, Smutok, Oleh, Johnston, Wayne A., Ayva, Cagla Ergun, Walden, Patricia, McWhinney, Brett, Ungerer, Jacobus P.J., Melman, Artem, Katz, Evgeny, & Alexandrov, Kirill (2022) Circular Permutated PQQ-Glucose Dehydrogenase as an Ultrasensitive Electrochemical Biosensor. Angewandte Chemie - International Edition, 61(6), Article number: e202109005.

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Lo, Harriet P., Lim, Ye Wheen, Xiong, Zherui, Martel, Nick, Ferguson, Charles, Ariotti, Nicholas, Giacomotto, Jean, Rae, James, Floetenmeyer, Matthias, Moradi, Shayli Varasteh, Gao, Ya, Tillu, Vikas A., Xia, Di, Wang, Huang, Rahnama, Samira, Nixon, Susan J., Bastiani, Michele, Day, Ryan D., Smith, Kelly A., Palpant, Nathan J., Johnston, Wayne A., Alexandrov, Kirill, Collins, Brett M., Hall, Thomas E., & Parton, Robert G. (2021) Cavin4 interacts with Bin1 to promote T-tubule formation and stability in developing skeletal muscle. Journal of Cell Biology, 220(12), Article number: e201905065.

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Guo, Zhong, Smutok, Oleh, Johnston, Wayne A., Walden, Patricia, Ungerer, Jacobus P.J., Peat, Thomas S., Newman, Janet, Parker, Jake, Nebl, Tom, Hepburn, Caryn, Melman, Artem, Suderman, Richard J., Katz, Evgeny, & Alexandrov, Kirill (2021) Design of a methotrexate-controlled chemical dimerization system and its use in bio-electronic devices. Nature Communications, 12, Article number: 7137.

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Varasteh Moradi, Shayli, Johnston, Wayne A., Parker, Jake, & Alexandrov, Kirill (2021) Leishmania tarentolae cell-free based approach for rapid anitbody-antigen interaction analysis. In Kelman, Zvi & O'Dell, William B. (Eds.) Recombinant Protein Expression: Prokaryotic Hosts and Cell-Free Systems. Academic Press, United States of America, pp. 391-409.

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Souilmi, Yassine, Lauterbur, M. Elise, Tobler, Ray, Huber, Christian D., Johar, Angad S., Moradi, Shayli Varasteh, Johnston, Wayne A., Krogan, Nevan J., Alexandrov, Kirill, & Enard, David (2021) Erratum: An ancient viral epidemic involving host coronavirus interacting genes more than 20,000 years ago in East Asia. Current Biology, 31(16), p. 3704.

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Souilmi, Yassine, Lauterbur, M. Elise, Tobler, Ray, Huber, Christian D., Johar, Angad S., Moradi, Shayli Varasteh, Johnston, Wayne A., Krogan, Nevan J., Alexandrov, Kirill, & Enard, David (2021) An ancient viral epidemic involving host coronavirus interacting genes more than 20,000 years ago in East Asia. Current Biology, 31(16), 3504-3514.e9.

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Cui, Zhenling, Johnston, Wayne A., & Alexandrov, Kirill (2020) Cell-Free Approach for Non-canonical Amino Acids Incorporation Into Polypeptides. Frontiers in Bioengineering and Biotechnology, 8, Article number: 1031.

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Edwardraja, Selvakumar, Guo, Zhong, Whitfield, Jason, Lantadilla, Ignacio Retamal, Johnston, Wayne A., Walden, Patricia, Vickers, Claudia E., & Alexandrov, Kirill (2020) Caged Activators of Artificial Allosteric Protein Biosensors. ACS Synthetic Biology, 9(6), pp. 1306-1314.

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Varasteh Moradi, Shayli, Gagoski, Dejan, Mureev, Sergey, Walden, Patricia, McMahon, Kerrie Ann, Parton, Robert G., Johnston, Wayne A., & Alexandrov, Kirill (2020) Mapping Interactions among Cell-Free Expressed Zika Virus Proteins. Journal of Proteome Research, 19(4), pp. 1522-1532.

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Das Gupta, Kaustav, Shakespear, Melanie R, Curson, James E B, Murthy, Ambika M V, Iyer, Abishek, Hodson, Mark P, Ramnath, Divya, Tillu, Vikas A, von Pein, Jessica B, Reid, Robert C, Tunny, Kathryn, Hohenhaus, Daniel M, Moradi, Shayli Varasteh, Kelly, Gregory M, Kobayashi, Takumi, Gunter, Jennifer H, Stevenson, Alexander J, Xu, Weijun, Luo, Lin, Jones, Alun, Johnston, Wayne A, Blumenthal, Antje, Alexandrov, Kirill, Collins, Brett M, Stow, Jennifer L, Fairlie, David P, & Sweet, Matthew J (2020) Class IIa histone deacetylases drive toll-like receptor-inducible glycolysis and macrophage inflammatory responses via pyruvate kinase M2. Cell Reports, 30(8), 2712-2728.e8.

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Guo, Zhong, Johnston, Wayne, Whitfield, Jason, Walden, Patricia, Cui, Zhenling, Wijker, Elvira, Edwardraja, Selvakumar, Retamal Lantadilla, Ignacio, Ely, Fernanda, Vickers, Claudia, Ungerer, Jacobus, & Alexandrov, Kirill (2019) Generalizable protein biosensors based on synthetic switch modules. Journal of the American Chemical Society, 141(20), pp. 8128-8135.

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Johnston, Wayne, Varasteh Moradi, Shayli, & Alexandrov, Kirill (2019) Adaption of the Leishmania cell-free expression system to high-throughput analysis of protein interactions. Methods in Molecular Biology, 2025, pp. 403-421.

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Jung, WooRam, Sierecki, Emma, Bastiani, Michele, O'Carroll, Ailis, Alexandrov, Kirill, Rae, James, Johnston, Wayne, Hunter, Dominic, Ferguson, Charles, Gambin, Yann, Ariotti, Nicholas, & Parton, Robert (2018) Cell-free formation and interactome analysis of caveolae. Journal of Cell Biology, 217(6), pp. 2141-2165.

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Gagoski, Dejan, Shi, Zhenyu, Nielsen, Lars, Vickers, Claudia, Mahler, Stephen, Speight, Robert, Johnston, Wayne, & Alexandrov, Kirill (2017) Cell-free pipeline for discovery of thermotolerant xylanases and endo-1,4-Beta-glucanases. Journal of Biotechnology, 259, pp. 191-198.

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Hussein, Waleed, Choi, Phil, Zhang, Cheng, Su, Mei, Sierecki, Emma, Johnston, Wayne, Fagan, Vincent, Alexandrov, Kirill, Skwarczynski, Mariusz, Gambin, Yann, Toth, Istvan, & Simerska, Pavla (2017) Evaluation of lipopeptides as toll-like receptor 2 ligands. Current Drug Delivery, 14(7), pp. 935-943.

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Overman, Jeroen, Fontaine, Frank, Moustaqil, Mehdi, Mittal, Deepak, Sierecki, Emma, Sacilotto, Natalia, Zuegg, Johannes, Robertson, Avril, Holmes, Kelly, Salim, Angela, Mamidyala, Sreeman, Butler, Mark, Robinson, Ashley, Lesieur, Emmanuelle, Johnston, Wayne, Alexandrov, Kirill, & other, and (2017) Pharmacological targeting of the transcription factor SOX18 delays breast cancer in mice. eLife, 6, Article number: e21221 1-18.

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Huang, Weiliang, Johnston, Wayne A., Boden, Mikael, & Gillam, Elizabeth M.J. (2016) ReX: A suite of computational tools for the design, visualization, and analysis of chimeric protein libraries. BioTechniques, 60(2), pp. 91-94.

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Guo, Zhong, Murphy, Lindy, Stein, Viktor, Johnston, Wayne, & Alexandrov, Kirill (2016) Engineered PQQ-glucose dehydrogenase as a universal biosensor platform. Journal of the American Chemical Society, 138(32), pp. 10108-10111.

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Guo, Zhong, Johnston, Wayne, Stein, Viktor, Kalimuthu, Palraj, Perez-Alcala, Siro, Bernhardt, Paul, & Alexandrov, Kirill (2016) Engineering PQQ-glucose dehydrogenase into an allosteric electrochemical Ca2+ sensor. Chemical Communications, 52(3), pp. 485-488.

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Gagoski, Dejan, Polinkovsky, Mark, Mureev, Sergey, Kunert, Anne, Johnston, Wayne, Gambin, Yann, & Alexandrov, Kirill (2016) Performance benchmarking of four cell-free protein expression systems. Biotechnology and Bioengineering, 113(2), pp. 292-300.

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Kubala, Marta, Norwood, Suzanne, Gomez, Guillermo, Jones, Alun, Johnston, Wayne, Yap, Alpha, Mureev, Sergey, & Alexandrov, Kirill (2015) Mammalian farnesyltransferase alpha-subunit regulates vacuolar protein sorting-associated protein 4A (Vps4A) - dependent intracellular trafficking through recycling endosomes. Biochemical and Biophysical Research Communications, 468(4), pp. 580-586.

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Alexandrov, Kirill & Johnston, Wayne (Eds.) (2014) Cell-free Protein Synthesis: Methods and Protocols. Methods in Molecular Biology. Humana Press, Totowa.

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Han, Siew Ping, Gambin, Yann, Gomez, Guillermo, Verma, Suzie, Giles, Nichole, Michael, Magdalene, Wu, Selwin, Guo, Zhong, Johnston, Wayne, Sierecki, Emma, Parton, Robert, Alexandrov, Kirill, & Yap, Alpha (2014) Cortactin scaffolds Arp2/3 and WAVE2 at the epithelial zonula adherens. Journal of Biological Chemistry, 289(11), pp. 7764-7775.

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Martin, Sally, Papadopulos, Andreas, Tomatis, Vanesa, Sierecki, Emma, Malintan, Nancy, Gormal, Rachel, Giles, Nichole, Johnston, Wayne, Alexandrov, Kirill, Gambin, Yann, Collins, Brett, & Meunier, Frederic (2014) Increased polyubiquitination and proteasomal degradation of a Munc18-1 disease-linked mutant causes temperature-sensitive defect in exocytosis. Cell Reports, 9(1), pp. 206-218.

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Johnston, Wayne & Alexandrov, Kirill (2014) Production of eukaryotic cell-free lysate from Leishmania tarentolae. Methods in Molecular Biology, 1118, pp. 1-15.

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Sierecki, Emma, Stevers, Loes, Giles, Nichole, Polinkovsky, Mark, Moustaqil, Mehdi, Mureev, Sergey, Johnston, Wayne, Dahmer-Heath, Mareike, Skalamera, Duka, Gonda, Thomas, Gabrielli, Brian, Collins, Brett, Alexandrov, Kirill, & Gambin, Yann (2014) Rapid mapping of interactions between human SNX-BAR proteins measured in vitro by alphascreen and single-molecule spectroscopy. Molecular and Cellular Proteomics, 13(9), pp. 2233-2245.

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Behrendorff, James B.Y.H., Johnston, Wayne A., & Gillam, Elizabeth M.J. (2014) Restriction enzyme-mediated DNA family shuffling. In Gillam, Elizabeth M.J., Copp, Janine N., & Ackerley, David (Eds.) Directed Evolution Library Creation: Methods and Protocols. [2nd ed.]. Humana Press, New York, NY, pp. 175-187.

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Gambin, Yann, Ariotti, Nicholas, McMahon, Kerrie-Ann, Bastiani, Michele, Sierecki, Emma, Kovtun, Oleksiy, Polinkovsky, Mark, Magenau, Astrid, Jung, WooRam, Okano, Satomi, Zhou, Yong, Leneva, Natalya, Mureev, Sergey, Johnston, Wayne, Gaus, Katharina (Kat), Hancock, John, Collins, Brett, Alexandrov, Kirill, & Parton, Robert (2014) Single-molecule analysis reveals self assembly and nanoscale segregation of two distinct cavin subcomplexes on caveolae. eLife, 3, Article number: e01434 1-18.

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Kovtun, Oleksiy, Tillu, Vikas, Jung, WooRam, Leneva, Natalya, Ariotti, Nicholas, Chaudhary, Natasha, Mandyam, Ramya, Ferguson, Charles, Morgan, Garry, Johnston, Wayne, Harrop, Stephen, Alexandrov, Kirill, Parton, Robert, & Collins, Brett (2014) Structural insights into the organization of the Cavin membrane coat complex. Developmental Cell, 31(4), pp. 405-419.

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Behrendorff, James B.Y.H., Johnston, Wayne A., & Gillam, Elizabeth M.J. (2013) DNA shuffling of cytochrome P450 enzymes. In Phillips, Ian R., Shephard, Elizabeth A., & de Montellano, Paul R. Ortiz (Eds.) Cytochrome P450 Protocols. [3rd ed.]. Humana Press, New York, NY, pp. 177-188.

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Johnston, Wayne A. & Gillam, Elizabeth M.J. (2013) Measurement of P450 difference spectra using intact cells. In Phillips, Ian R., Shephard, Elizabeth A., & de Montellano, Paul R. Ortiz (Eds.) Cytochrome P450 Protocols. [3rd ed.]. Humana Press, New York, NY, pp. 189-204.

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Guo, Zhong, Johnston, Wayne, Kovtun, Oleksiy, Mureev, Sergey, Broecker, Cornelia, Ungermann, Christian, & Alexandrov, Kirill (2013) Subunit organisation of in vitro reconstituted HOPS and CORVET multisubunit membrane tethering complexes. PLoS One, 8(12), Article number: e81534 1-9.

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Behrendorff, James B.Y.H., Moore, Chad D., Kim, Keon Hee, Kim, Dae Hwan, Smith, Christopher A., Johnston, Wayne A., Yun, Chul Ho, Yost, Garold S., & Gillam, Elizabeth M.J. (2012) Directed evolution reveals requisite sequence elements in the functional expression of P450 2F1 in Escherichia coli. Chemical Research in Toxicology, 25(9), pp. 1964-1974.

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Johnston, Wayne A., Hunter, Dominic J.B., Noble, Christopher J., Hanson, Graeme R., Stok, Jeanette E., Hayes, Martin A., De Voss, James J., & Gillam, Elizabeth M.J. (2011) Cytochrome P450 is present in both ferrous and ferric forms in the resting state within intact Escherichia coli and hepatocytes. Journal of Biological Chemistry, 286(47), pp. 40750-40759.

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Hunter, D. J.B., Behrendorff, J. B.Y.H., Johnston, W. A., Hayes, P. Y., Huang, W., Bonn, B., Hayes, M. A., De Voss, J. J., & Gillam, E. M.J. (2011) Facile production of minor metabolites for drug development using a CYP3A shuffled library. Metabolic Engineering, 13(6), pp. 682-693.

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Kovtun, Oleksiy, Mureev, Sergey, Jung, WooRam, Kubala, Marta, Johnston, Wayne, & Alexandrov, Kirill (2011) Leishmania cell-free protein expression system. Methods, 55(1), pp. 58-64.

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Kovtun, Oleksiy, Mureev, Sergey, Johnston, Wayne, & Alexandrov, Kirill (2010) Towards the construction of expressed proteomes using a Leishmania tarentolae based cell-free expression system. PLoS One, 5(12), Article number: e14388 1-11.

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Johnston, Wayne A., Weiliang Huang, Huang, De Voss, James J., Hayes, Martin A., & Gillam, Elizabeth M.J. (2008) Quantitative whole-cell cytochrome P450 measurement suitable for high-throughput application. Journal of Biomolecular Screening, 13(2), pp. 135-141.

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Johnston, Wayne A., Huang, Weiliang, De Voss, James J., Hayes, Martin A., & Gillam, Elizabeth M.J. (2007) A shuffled CYP1A library shows both structural integrity and functional diversity. Drug Metabolism and Disposition, 35(12), pp. 2177-2185.

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Huang, Weiliang, Johnston, Wayne A., Hayes, Martin A., De Voss, James J., & Gillam, Elizabeth M.J. (2007) A shuffled CYP2C library with a high degree of structural integrity and functional versatility. Archives of Biochemistry and Biophysics, 467(2), pp. 193-205.

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Rosic, Nedeljka N., Huang, Weiliang, Johnston, Wayne A., DeVoss, James J., & Gillam, Elizabeth M.J. (2007) Extending the diversity of cytochrome P450 enzymes by DNA family shuffling. Gene, 395(1-2), pp. 40-48.

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Cooney, Michael J., Johnston, Wayne A., Pohl, Silke, & Bidigare, Robert R. (2006) Influence of photoperiod on pigmentation and metabolic efficiency of the marine aerobic anoxygenic photosynthetic bacterium Erythrobacter longus Strain NJ3Y. Aquatic Microbial Ecology, 43(3), pp. 303-309.

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Johnston, Wayne, Maynard, Nathan, Liaw, Bor Yann, & Cooney, Michael J. (2006) In situ measurement of activity and mass transfer effects in enzyme immobilized electrodes. Enzyme and Microbial Technology, 39(1), pp. 131-140.

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Johnston, Wayne, Cooney, Michael, Schorlemmer, Anita, Pohl, Silke, Karl, David M., & Bidigare, Robert (2005) Carbon mass balance methodology to characterize the growth of pigmented marine bacteria under conditions of light cycling. Bioprocess and Biosystems Engineering, 27(3), pp. 163-174.

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Johnston, Wayne, Cooney, Michael J., Liaw, Bor Yann, Sapra, Rajat, & Adams, Michael W. W. (2005) Design and characterization of redox enzyme electrodes: New perspectives on established techniques with application to an extremeophilic hydrogenase. Enzyme and Microbial Technology, 36(4), pp. 540-549.

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Cooney, Michael, Liaw, Bor Yann, Johnston, Wayne, Svoboda, Vojtech, Quinlan, Forest, & Maynard, Nathan (2005) Design of macropore structure for enzyme fuel cells operation. ACS Division of Fuel Chemistry, Preprints, 50(2), pp. 630-631.

Johnston, Wayne A., Stewart, Meredith, Lee, Peter, & Cooney, Michael J. (2003) Tracking the acetate threshold using DO-transient control during medium and high cell density cultivation of recombinant Escherichia coli in complex media. Biotechnology and Bioengineering, 84(3), pp. 314-323.

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Johnston, Wayne A. & Cooney, Michael J. (2003) Broth recycling to reduce process noise resulting from concentrated substrate addition in fed-batch cultivation of Escherichia coli. Biotechnology Progress, 19(2), pp. 659-661.

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Davis, Timothy M.E., Binh, Tran Quang, Thu, Le Thi Anh, Long, Ton That Ai, Johnston, Wayne, Robertson, Ken, & Barrett, P. Hugh R. (2002) Glucose and lactate turnover in adults with falciparum malaria: Effect of complications and antimalarial therapy. Transactions of the Royal Society of Tropical Medicine and Hygiene, 96(4), pp. 411-417.

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Johnston, W., Cord-Ruwisch, R., & Cooney, M. J. (2002) Industrial control of recombinant E. coli fed-batch culture: New perspectives on traditional controlled variables. Bioprocess and Biosystems Engineering, 25(2), pp. 111-120.

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Singh, Balbir, Choo, Keng Ee, Ibrahim, Jamal, Johnston, Wayne, & Davis, Timothy M.E. (1998) Non-radioisotopic glucose turnover in children with falciparum malaria and enteric fever. Transactions of the Royal Society of Tropical Medicine and Hygiene, 92(5), pp. 532-537.

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Binh, T. Q., Davis, T. M.E., Johnston, W., Thu, L. T.A., Boston, R., Danh, P. T., & Anh, T. K. (1997) Glucose metabolism in severe malaria: Minimal model analysis of the intravenous glucose tolerance test incorporating a stable glucose label. Metabolism: Clinical and Experimental, 46(12), pp. 1435-1440.

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Skinner-Adams, Tina S., Davis, Timothy M.E., Manning, Linda S., & Johnston, Wayne A. (1997) The efficacy of benzimidazole drugs against Plasmodium falciparum in vitro. Transactions of the Royal Society of Tropical Medicine and Hygiene, 91(5), pp. 580-584.

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Skinner, Tina S., Manning, Linda S., Johnston, Wayne A., & Davis, Timothy M.E. (1996) In vitro stage-specific sensitivity of Plasmodium falciparum to quinine and artemisinin drugs. International Journal for Parasitology, 26(5), pp. 519-525.

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